Characteristics of natural antibody-secreting cells

Intestinal Extravasation of IgA Antibody-Secreting Cells

Microfluidic Trapping of Antibody-secreting Cells

The development of antibody-based drugs typically begins with the isolation and screening of thousands of antibody-secreting cells, often requiring 4-6 weeks to identify only 1-10 cell lines of interest. We are developing a novel immunobiosensing device to afford direct and rapid assessment of the antibody production of each of thousands of living cells captured on a single slide. Each cell sha...

Age-related changes in the transcriptome of antibody-secreting cells

We analyzed age-related defects in B cell populations from young and aged mice. Microarray analysis of bone marrow resident antibody secreting cells (ASCs) showed significant changes upon aging, affecting multiple genes, pathways and functions including those that play a role in immune regulation, humoral immune responses, chromatin structure and assembly, cell metabolism and the endoplasmic re...

Origin and Homing of Intestinal IgA Antibody-secreting Cells

The fact that IgA comprises the body's major isotype of antibody on a biosynthetic basis is not widely appreciated because IgG, not IgA, is the predominant isotype in serum. Nevertheless, the bulk of the body's Ig-producing cells reside in the various mucosal and exocrine sites, especially along the intestinal tract, and most of them make IgA anti-bodies against environmental antigens, includin...

Antibody-secreting plasma cells persist for decades in human intestine

Plasma cells (PCs) produce antibodies that mediate immunity after infection or vaccination. In contrast to PCs in the bone marrow, PCs in the gut have been considered short lived. In this study, we studied PC dynamics in the human small intestine by cell-turnover analysis in organ transplants and by retrospective cell birth dating measuring carbon-14 in genomic DNA. We identified three distinct...